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A Alkaline phosphatase staining. B Quantification of alkaline phosphatase enzyme activity. C Osteogenic gene expression following infection <t>with</t> <t>Adv-BMP9</t> or control virus in the presence of 10 ng/ml TGFβ1 ( n = 3). D Phosphorylation of Smads in marrow stromal cells following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1, as detected by western blot. E BMP-pSmad1/5/8 signaling target gene expression in marrow stromal cells following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1 ( n = 3). ns no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Adv Bmp9, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A Alkaline phosphatase staining. B Quantification of alkaline phosphatase enzyme activity. C Osteogenic gene expression following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1 ( n = 3). D Phosphorylation of Smads in marrow stromal cells following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1, as detected by western blot. E BMP-pSmad1/5/8 signaling target gene expression in marrow stromal cells following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1 ( n = 3). ns no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: NPJ Microgravity

Article Title: BMP9 attenuates microgravity-related disuse osteoporosis by modulating TGFβ and BMP signaling

doi: 10.1038/s41526-025-00510-y

Figure Lengend Snippet: A Alkaline phosphatase staining. B Quantification of alkaline phosphatase enzyme activity. C Osteogenic gene expression following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1 ( n = 3). D Phosphorylation of Smads in marrow stromal cells following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1, as detected by western blot. E BMP-pSmad1/5/8 signaling target gene expression in marrow stromal cells following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1 ( n = 3). ns no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: For chondrogenic differentiation, after infection with Adv-BMP9 or Adv-EGFP, TGFβ3 (10 ng/mL, Topscience, China) was added to F12/DMEM 1:1 (Gibco, USA) containing 10% fetal bovine serum (Shanghai Sangon, China) and 1% penicillin-streptomycin (Solarbio, China).

Techniques: Staining, Activity Assay, Gene Expression, Infection, Control, Virus, Phospho-proteomics, Western Blot, Targeted Gene Expression

A Phosphorylation of Smads in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1, as detected by western blot. B BMP-pSmad1/5/8 signaling target gene expression in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1 ( n = 3). C Intracellular pH changes in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1, normalized to control group. D Expression of osteoclastic genes in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1 ( n = 3). ns no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: NPJ Microgravity

Article Title: BMP9 attenuates microgravity-related disuse osteoporosis by modulating TGFβ and BMP signaling

doi: 10.1038/s41526-025-00510-y

Figure Lengend Snippet: A Phosphorylation of Smads in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1, as detected by western blot. B BMP-pSmad1/5/8 signaling target gene expression in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1 ( n = 3). C Intracellular pH changes in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1, normalized to control group. D Expression of osteoclastic genes in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1 ( n = 3). ns no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: For chondrogenic differentiation, after infection with Adv-BMP9 or Adv-EGFP, TGFβ3 (10 ng/mL, Topscience, China) was added to F12/DMEM 1:1 (Gibco, USA) containing 10% fetal bovine serum (Shanghai Sangon, China) and 1% penicillin-streptomycin (Solarbio, China).

Techniques: Phospho-proteomics, Infection, Control, Virus, Western Blot, Targeted Gene Expression, Expressing